2019-04-16 14:31:012025-03-16 16:40:34
description
low affinity potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit
glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit
locus
BSU13500
BSU_13500
product
low affinity potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit
glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit
outlinks
bsu
BSU13500
BSU_13500
The protein
Kinetic information
the [[protein|KtrC]]-[[protein|KtrD ]]channel has a low affinity for potassium,this is determined by KtrD [pubmed|30753894]
the [[protein|KtrC]]-[[protein|KtrD ]]channel has a low affinity for potassium,this is determined by KtrD [pubmed|30753894]
the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate (from 0.278 mM to 0.17 mM) [pubmed|32253343]
Biological materials
Expression vector
pGP2996: (IPTG inducible expression of [[gene|ktrCD]], purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP2996: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
Biological materials
FLAG-tag construct
GP2434 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
GP3465 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]
[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]
[SW|João H Morais-Cabral], University of Porto, Portugal [https://www.i3s.up.pt/research-group?x=47 Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]
References
Original publications
The protein
Protein family
TrkH potassium transport family (with [[protein|KtrB]], according to UniProt)
The protein
Effectors of protein activity
the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate [pubmed|32253343]