SubtiBank SubtiBank
Version comparison:

2019-04-16 14:31:012025-03-16 16:40:34

description

low affinity potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit

glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit

locus

BSU13500

BSU_13500

product

low affinity potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit

glutamate-controlled potassium channel [[protein|KtrC]]-[[protein|KtrD]], integral membrane subunit

outlinks

bsu

BSU13500

BSU_13500

The protein

Kinetic information

the [[protein|KtrC]]-[[protein|KtrD ]]channel has a low affinity for potassium,this is determined by KtrD [pubmed|30753894]

the [[protein|KtrC]]-[[protein|KtrD ]]channel has a low affinity for potassium,this is determined by KtrD [pubmed|30753894]

the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate (from 0.278 mM to 0.17 mM) [pubmed|32253343]

Biological materials

Expression vector

pGP2996: (IPTG inducible expression of [[gene|ktrCD]], purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP2996: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

Biological materials

FLAG-tag construct

GP2434 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

GP3465 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]

[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]

[SW|João H Morais-Cabral], University of Porto, Portugal [https://www.i3s.up.pt/research-group?x=47 Homepage]

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]

References

Original publications

12562800, 23598340, 28420751, 28504641, 30753894

12562800, 23598340, 28420751, 28504641, 30753894, 32253343

The protein

Protein family

TrkH potassium transport family (with [[protein|KtrB]], according to UniProt)

The protein

Effectors of protein activity

the affinity of [[protein|KtrC]]-[[protein|KtrD]] for potassium is strongly increased in the presence of glutamate [pubmed|32253343]